Comparative Elimination of Begomoviruses in Cassava Meristems and Axillary Buds
Journal of Experimental Agriculture International,
Aim: The production of healthy cuttings from a local cassava cultivar for cassava mosaic control.
Study design: The study was carried out at the In Vitro Culture Laboratory using the techniques of thermotherapy and culture of tissues and explants in a specific medium.
Place and duration: The study was carried out at the Laboratory of Biological and Agronomic Sciences for Development at the University of Bangui, Central African Republic from December 2017 to June 2018.
Methodology: A variety of cassava called six-month very susceptible to mosaic was used for this work. The cuttings used were infected by Cassava Mosaic Begomoviruses (CMBs) with high severity. It were subcultured in a room under the heat of 37 ° C to 40 ° C for two weeks. Explants and meristems were taken from the stems and the apices, respectively. These collected materials were treated and seeded on appropriate culture media. After the plants produced in vitro were acclimatized and the leaves were removed to check their phytosanitary state by the PCR technique.
Results: The rate of emergence of the acclimatized plants and the expression of the disease on the microplants were evaluated. The results show that 75% of the weaned vitro plants recovered under acclimatization. In addition, the acclimatized plants left growing in the greenhouse for four months remained asymptomatic. Molecular analysis by PCR showed that begomoviruses were not detected on meristem samples unlike samples from stem fragments.
Conclusion: The combination of thermotherapy technique associated with the culture of meristems constitutes an effective means useful for the sanitation of infected plant material.
- Manihot esculenta
- in vitro culture
- central African Republic
How to Cite
Engelmann F. In vitro conservation of tropical plant germoplasm: a review. Euphytica. 1991;57: 227-243.
Mallouhi N, Kafara JM. La culture du manioc en Centrafrique. CTP/ICRA. 2002;16.
Tom C, Mity T. Pesticidal activity of the leaves of manihot esculenta against the pest tribolium castaneum . WJPMR. 2017;3(1):286-290.
Legg J, Owor BS, seruwagi P, Ndunguru J. Cassava mosaic virus disease in East and Central Africa epidemiology and management of a regional pandemic. Advances in Virus Research 2006; 67:355-418.
Patil BL, Fauquet CM. Cassava mosaic geminiviruses: Actual knowledge and perspectives. Molecular Plant Pathology. 2009;10:685-701.
Ndunguru J, Legg J, Aveling T, Thompson G, Fauquet C. Molecular biodiversity of cassava begomoviruses in Tanzania: evolution of cassava geminiviruses in Africa and evidence for East Africa being a center of diversity of cassava geminiviruses. Virology Journal. 2005;2:21.
Ndunguru J, Legg JP, Fofana IBF, Aveling TAS, Thompson G, Fauquet CM, Calvert L. Identification of a defective molecule derived from DNA-A of the bipartite begomovirus of East African cassava mosaic virus. Plant Pathology. 2006;55:2-10.
Legg J, Ogwal S. Changes in the incidence of African cassava mosaic virus disease and the abundance of its whitefly vector along south–north transects in Uganda. Journal of Applied Entomology. 1998;12:169-178.
Zinga I, Chiroleu F, Kamba E, Giraud-Carrier C, Harimalala1 M, Kosh Komba E, Yandia S, Semballa S, Reynaud B, Dintinger J, Lefeuvre P, Lett JM. Field Evaluation of the Effectiveness of Thermotherapy against Cassava Mosaic Disease in Central African Republic. American Journal of Experimental Agriculture. 2014;4(11):1232-1241.
Zinga I, Chiroleu F, Legg J, Lefeuvre P, Kosh Komba E, Semballa S, Yandia PS, Mandakombo NB, Reynaud B, Lett JM. Epidemiology assessement of cassava mosaic disease in Central African Republic reveals the importance of mixed viral infection and poor health of plant cutting. Crop Protection. 2013;4:6-12.
Zinga I, Yongo OD, Semballa S, Toukia IG, Kamba E, Konguere E, Mvila A, Bagofou Kounga YA, Pabamé NSS, Tchuanyo M, Ondo Bindang C, Pounaba N, Valam Zango A, Namkosserena S, Mouliom Pefoura A. Detection and geographical distribution of cassava begomoviruses in the six countries of Economic and Monetary Community of Central Africa. Science et Technique, Sciences naturelles Appliquées. SPECIAL SIST 2017 SNA2_agrono27i. 2018;251-260.
Sseruwagi PS, serubombwe WS, Legg JP, Ndunguru J. Thresh JM. Methods of surveying the incidence and whitefly vector populations on cassava in Africa: A review. Virus Res. 2004; 100:129e142.
Fauquet C, Fargette D. African cassava mosaic virus: etiology, epidemiology and control. Plant Disease. 1990;74:404- 411.
Mallowa SO, Isutsa DK, Kamau AW, Legg JP. Effectiveness of phytosanitation in cassava mosaic disease management in a postepidemic area of western Kenya. ARPN Journal of Agricultural and Biologicla Science. 2011;6(7).
Ummey H., Sharmin R., Mihir L.S., Khan M.R., Syed H.. Endogenous Bacterial Contamination During In vitro Culture of Table Banana: Identification and Prevention. Plant Tissue Cult. 2002; 12(2) :117-124.
FAOSTAT. FAO database. Food and Agriculture Organization of the United Nations, Rome Italy; 2016.
George EF. Plant propagation by tissue culture (Part 1). In The Technology (2nd edn). Exegetics Ltd. Edington; 1993a.
George EF. Plant propagation by tissue culture (Part 2). In Practice (2nd edn). Exegetics Ltd.: Edington. 1993b.
Feréol L. Multiplication végétative et élimination de la mosaïque du manioc par thermothérapie sur des plantes cultivées in vitro. In Diseases of tropical food crops. Proceedings of an International Symposium V.C.L. Louvain-La-Neuve. Belgium. 1978;285-295.
Yandia Simplice Prosper, Wouyou D. Agapit, Silla Semballa, Dambier Dominique, Gandonou Christophe Bernard and Toukourou Fatiou. Elimination of African cassava mosaic virus in cassava (Manihot esculenta Crantz) using meristem culture associated to thermotherapy. International Journal of Development Research. 2015;5(10):5655-5660.
Ng SY. Culture des tissus. Le manioc en Afrique tropical : un manuel de référence. IITA & UNICEF. 1990;51-61.
Mabanza J, Jonard R. La multiplication des clones de manioc à partir d'apex isolés in vitro. Comptes Rendus de C.R.A. Sciences. Paris, Tome 291, Série III. 1981;830-842.
Murashige T, Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 1962;15:473-497.
Otabo Françoise R, Mabanza Joseph. Sevrage et transfert en plein champ des vitro plants de manioc. Protocole de sevrage. Centre de recherches sur l’Amélioration Génétique des plantes tropicales (CERAG); 1996.
Delatte H, Martin DP, Naze F, Goldbach R, Reynaud B, Peterschmitt M, Lett J-M. South West Indian Ocean islands tomato begomovirus populations represent a new major monopartite begomovirus group. J. Gen. Virol. 2005 ;86:1533e 1542.
Cooper VC, Walkey DJA. Therminal inactivation of cherry leaf roll virus in tissue cultures of Nicotiana rustica raised from seeds and meristem tips. Ann. Appl. Biol. 1978;88:273-278.
Karta KK, Gamborg OC. Elimination of Cassava Mosaic Disease by meristem culture. Phytopatholoqy. 1975;65(7):826-828.
Kwabena A. Use of tissue culture and thermotherapy. Accra, GH, College of Agriculture and Natural Resources, PhD Thesis. Internet Resource.
Manganaris GA, Economou A, Boubourakas I, Katis N. Elimination of PPV and PNRSV through thermotherapy and meristem-tip culture in nectarine. Plant Cell Reports. 2003;22:195-200.
Mink GI, Wample R, Howell WE. Heat treatment of perennial plants to eliminate phytoplasms, viruses, and viroids while maintening plant survival. In : plant Virus Disease Control (Hadid A, Khetarpal RK, koganezawa H. eds). St. Paul, MN: APS Press, The American Phytopathological Society. 1998;332-345.
Badou BT, Pita J, Houedjissin SS, Corneille Ahannhanzo. Contrôle des germes endogènes par usage d’agents antimicrobiens et réponse de différents explants durant la phase d’initiation in vitro de l'ananas (Ananas comosus (L.), Mill var. comosus). Journal of Applied Biosciences. 2018;121:12129-12143.
Badou BT, Agbidinoukoun A, Nondichao L, Dossoukpèvi R, Cacaï GTH, Houédjissin SS, Ahanhanzo C. Effects of two disinfectants and two growth regulators on in vitro propagation of Smooth Cayenne and Sugarloaf cultivars of Pineapple (Ananas comosus (L) Mill var. comosus). Journal of Biotechnology Research. 2017;3(10):94-105
De Silva TCY, Fernando KMEP, Senerath WTPSK. Effect of incorporation of fungicides into tissue culture medium on fungal contaminants of in vitro grown Kaempferia galanga. University of Sri Jayewardenepura, Gangodawila, Nugegoda, Sri- Lanka; 2011.
Talukder MI, Begum F, Azad MMK. Management of pineapple disease of sugarcane through biological means. J. Agric. Rural Dev. 2007;5(1-2):79- 83.
Phillips R, Arnott SM, Kaplan SE. Antibiotics in plant tissue culture: Rifampicin effectively controls bacterial contaminants without affecting the growth of short-term explant cultures of Helianthus tuberosus. Plant Sci. Lett. 1981;21(3):235-240.
Abstract View: 307 times
PDF Download: 195 times