In vitro Effect of Essential Oil of Peppermint (Mentha x piperita L.) on the Mycelial Growth of Alternaria alternata

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K. R. S. França
T. L. Silva
T. A. L. Cardoso
A. L. N. Ugulino
A. P. M. Rodrigues
A. F. de Mendonça Júnior


Aims: The research aimed to evaluate the in vitro antifungal effect of the essential oil of peppermint (Mentha x piperita L.) in the control of Alternaria alternata.

Study Design: The experiment was conducted in a completely randomised experimental design with five treatments in four replicates each.

Place and Duration: The work was conducted at the Center of Science and Technology Agri-food of the Federal University of Campina Grande, Pombal-PB, Brazil, between February and March of 2018.

Methodology: The essential oil was added to the PDA culture medium (Potato-Dextrose-Agar) autoclaved and subsequently poured into Petri plates. The treatments comprised five concentrations of the oil (0.0, 0.4, 0.6, 0.8, and 1.0%). After the inoculation with fungi, the plates were incubated for 14 days in a B.O.D incubator at 27±2°C. With the data of mycelial diameters, the percentage of mycelial growth inhibition (PGI) and index of mycelial growth speed (IMGS) were calculated.

Results: All concentrations of peppermint oil reduced the mycelial growth of A. alternata. The minimum and maximum inhibitions occurred in the concentrations of 0.4 and 0.8%, which reached -13.27 and 72.45%, respectively. Although the maximum inhibition was 72.45%, the average percentages were 41.67 and 37.18% at the highest concentrations, showing an intermediate power of inhibition. A dose-dependent behaviour, was observed which suggests that further increases in concentration may enhance the inhibition effect of the oil.

Conclusion: The peppermint oil can be used as a viable and sustainable antifungal product in the control of this pathogen. Due to the possible dose-dependent effect, the development of studies is recommended with concentrations around 2.25% to test if the oil reaches the total inhibition. The in vivo experiments are required to verify the oil feasibility on the control of A. alternata on plants and fruits.

Antifungal, disease control, fungitoxic effect, phytopathogens, postharvest diseases

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How to Cite
França, K. R. S., Silva, T. L., Cardoso, T. A. L., Ugulino, A. L. N., Rodrigues, A. P. M., & Júnior, A. F. de M. (2018). In vitro Effect of Essential Oil of Peppermint (Mentha x piperita L.) on the Mycelial Growth of Alternaria alternata. Journal of Experimental Agriculture International, 26(5), 1-7.
Original Research Article